Leu in the insulin B chain, Ser12Leu in the A chain, and IleGly, ProAla, and SerTrp in melittin
Reaction: Cleavage of only the single bond Ala14Leu in the insulin B chain, Ser12Leu in the A chain, and IleGly, ProAla, and SerTrp in melittin
Other names: Crotalus horridus metalloendopeptidase; hemorrhagic proteinase IV; Crotalus horridus horridus venom hemorrhagic proteinase
Comments: A 56 kDa hemorrhagic endopeptidase from the venom of the timber rattlesnake (Crotalus horridus horridus) that cleaves basement membrane, hide powder and fibrinogen.
Links to other databases: BRENDA, EXPASY, KEGG, MEROPS, Metacyc, CAS registry number: 84056-81-5
References:
1. Civello, D.J., Duong, H.L. and Geren, C.R. Isolation and characterization of a hemorrhagic proteinase from timber rattlesnake venom. Biochemistry 22 (1983) 749-755. [PMID: 6340728]
2. Civello, D.J., Moran, J.B. and Geren, C.R. Substrate specificity of a hemorrhagic proteinase from timber rattlesnake venom. Biochemistry 22 (1983) 755-762. [PMID: 6340729]