Leu and Ala14Leu in the insulin B chain
Reaction: Cleavage of only two bonds His10Leu and Ala14Leu in the insulin B chain
Other names: Trimeresurus metalloendopeptidase I; hemorrhagic proteinase HR1A; hemorrhagic metalloproteinase HR1A; metalloproteinase HR1A
Comments: A 60 kDa hemorrhagic endopeptidase of pI 4.4 from the venom of the habu snake (Trimeresurus flavoviridis). In peptidase family M12 (astacin family)
Links to other databases: BRENDA, EXPASY, KEGG, MEROPS, Metacyc, CAS registry number: 151125-16-5
References:
1. Omori-Satoh, T. and Sadahiro, S. Resolution of the major hemorrhagic component of Trimeresurus flavoviridis venom into two parts. Biochim. Biophys. Acta 580 (1979) 392-404. [PMID: 518906]
2. Yamakawa, Y. and Omori-Satoh, T. The sites of cleavage in oxidized insulin-B chain by a hemorrhagic protease derived from the venom of the habu (Trimeresurus flavoviridis). Toxicon 26 (1988) 227-231 .. [PMID: 3284004]
3. Takeya, H., Oda, K., Miyata, T., Omori-Satoh, T. and Iwanaga, S. The complete amino acid sequence of the high molecular mass hemorrhagic protein HR1B isolated from the venom of Trimeresurus flavoviridis. J. Biol. Chem. 265 (1990) 16068-16073. [PMID: 2398046]