Ala-Val-. The specificity further depends on the organization of a β-turn-α-helix of nine or more residues containing the paired basic amino acids near the centre [3]
Reaction: Hydrolysis of polypeptides with Arg or Lys in P1 and P2, e.g. to hydrolyse pro-oxytocin at -Lys-ArgAla-Val-. The specificity further depends on the organization of a β-turn-α-helix of nine or more residues containing the paired basic amino acids near the centre [3]
Other names: bovine neurosecretory granule protease cleaving pro-oxytocin/neurophysin; pro-oxytocin/neurophysin convertase; prooxyphysin proteinase; pro-oxytocin convertase
Comments: An endopeptidase of 58 kDa known from bovine pituitary neurosecretory granules and bovine and human corpus luteum [4,5]. Inhibited by EDTA [1]
Links to other databases: BRENDA, EXPASY, KEGG, MEROPS, Metacyc, CAS registry number: 162875-09-4
References
1. Clamagirand, C., Creminon, C., Fahy, C., Boussetta, H. and Cohen, P. Partial purification and functional properties of an endoprotease from bovine neurosecretory granules cleaving proocytosin/neurophysin peptides at the basic amino acid doublet. Biochemistry 26 (1987) 6018-6023. [PMID: 2825769]
2. Créminon, C., Rholam, M., Boussetta, H., Marrakchi, N. and Cohen, P. Synthetic peptide substrates as models to study a pro-ocytocin/neurophysin converting enzyme. J. Chromatogt. 440 (1988) 439-448. [PMID: 3042797]
3. Brakch, N., Boussetta, H., Rholam, M. and Cohen, P. Processing endoprotease recognizes a structural feature at the cleavage site of peptide prohormones. The pro-ocytocin/neurophysin model. J. Biol. Chem. 264 (1989) 15912-15916. [PMID: 2674120]
4. Plevrakis, I, Créminon, C., Clamagirand, C., Brakch, N. Rholam, M. and Cohen, P. Proocytocin/neurophysin convertase from bovine neurohypophysis and corpus luteum secretory granules: complete purification, structure-function relationships, and competitive inhibitor. Biochemistry 28 (1989) 2705-2710. [PMID: 2659078]
5. Guillou, M.D., Camier, M. and Clamagirand, C. Evidence for the presence of pro-oxytocin/neurophysin converting enzyme in the human ovary. J. Endocrinol. 142 (1994) 345-352. [PMID: 7931007]