EC 3.4.24.73

Reaction: Hydrolysis of -His¹⁰Leu-, -Ala¹⁴Leu-, -Tyr¹⁶Leu-and -Phe²⁴Phe- bonds in insulin B chain

Other names: HF₂-proteinase; JF1

Comments: Hemorrhagic endopeptidase from the venom of the jararaca snake (Bothrops jararaca). The 52-kDa enzyme contains a disintegrin domain [3]. In peptidase family M12 (astacin family)

Links to other databases: BRENDA, EXPASY, KEGG, MEROPS, Metacyc, PDB, CAS registry number: 160477-79-2

References

1. Mandelbaum, F.R., Reichl, A.P. and Assakura, M.T. Some physical and biochemical characteristics of HF₂, one of the hemorrhagic factors in the venom of Bothrops jararaca. In Animal, Plant and Microbial Toxins (Ohsaka, A., Hayashi, K. and Sawai, Y. eds), pp. 111-121 (1976) Plenum Press, New York

2. Assakura, M.T., Reichl, A.P. and Mandelbaum, F.R. Comparison of immunological, biochemical and biophysical properties of three hemorrhagic factors isolated from the venom of Bothrops jararaca (jararaca). Toxicon 24 (1986) 943-946. [PMID: 3810664]

3. Paine, M.J.I., Desmond, H.P., Theakston, R.D.G. and Crampton, J.M. Purification, cloning, and molecular characterization of a high molecular weight hemorrhagic metalloprotease, jararhagin, from Bothrops jararaca venom. Insights into the disintegrin gene family. J. Biol. Chem. 267 (1992) 22869-22876. [PMID: 1385408]