Val-Arg-Ser-Ser-Ser in the membrane-bound, 26-kDa form of tumour necrosis factor α (TNFα). Similarly cleaves other membrane-anchored, cell-surface proteins to "shed" the extracellular domains
Reaction: Narrow endopeptidase specificity. Cleaves Pro-Leu-Ala-Gln-AlaVal-Arg-Ser-Ser-Ser in the membrane-bound, 26-kDa form of tumour necrosis factor α (TNFα). Similarly cleaves other membrane-anchored, cell-surface proteins to "shed" the extracellular domains
Other names: tumor necrosis factor α-converting enzyme; TACE
Comments: In peptidase family M12. In vivo, the cleavage of tumor necrosis factor α precursor releases the soluble, 17-kDa TNFα, which induces inflammation.
Links to other databases: BRENDA, EXPASY, KEGG, MEROPS, Metacyc, PDB, CAS registry number: 151769-16-3
References:
1. Black, R.A. Tumor necrosis factor-α converting enzyme. Int. J. Biochem. Cell Biol. 34 (2002) 1-5. [PMID: 11733179]