Reaction: Hydrolyses the peptide bond -P2-(S-farnesyl or geranylgeranyl)C-P1'-P2'-P3'-COOH where where P1' and P2' are amino acids with aliphatic sidechains and P3' is any C-terminal residue
Other name(s): CaaX prenyl protease 2; prenyl protein-specific endoprotease 2; PPSEP 2; α-factor-converting enzyme RCE1; ras converting enzyme; RACE; glutamic-type intramembrane endopeptidase Rce1; type II CAAX proteas.
Comments: The cleavage site motif is typically referred to as CaaX, where the letter a represents any amino acid, rather than alanine, and X represents the C-terminal amino acid of the target protein. The enzyme has been found in the yeast Saccharomyces cerevisiae and homologues exist in humans and several other species. Although the cleavage site is similar to that of the metallo-peptidase Ste24 endopeptidase (EC 3.4.24.84), there appear to be specificity differences in the proteins hydrolysed by these two enzymes, with amino-acid substitution studies indicating activity of the yeast enzyme towards substrates with a hydrophylic residue at (P1') that are not hydrolysed by EC 3.4.24.84 [4].
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
References:
1. Otto, J.C., Kim, E., Young, S.G. and Casey, P.J. Cloning and characterization of a mammalian prenyl protein-specific protease. J. Biol. Chem. 274 (1999) 8379-8382. [PMID: 10085068]
2. Manolaridis, I., Kulkarni, K., Dodd, R.B., Ogasawara, S., Zhang, Z., Bineva, G., Reilly, N.O., Hanrahan, S.J., Thompson, A.J., Cronin, N., Iwata, S. and Barford, D. Mechanism of farnesylated CAAX protein processing by the intramembrane protease Rce1. Nature 504 (2013) 301-305. [PMID: 24291792]
3. Pei, J., Mitchell, D.A., Dixon, J.E. and Grishin, N.V. Expansion of type II CAAX proteases reveals evolutionary origin of γ-secretase subunit APH-1. J. Mol. Biol. 410 (2011) 18-26. [PMID: 21570408]
4. Trueblood, C.E., Boyartchuk, V.L., Picologlou, E.A., Rozema, D., Poulter, C.D. and Rine, J. The CaaX proteases, Afc1p and Rce1p, have overlapping but distinct substrate specificities. Mol. Cell Biol. 20 (2000) 4381-4392. [PMID: 10825201]
5. Plummer, L.J., Hildebrandt, E.R., Porter, S.B., Rogers, V.A., McCracken, J. and Schmidt, W.K. Mutational analysis of the ras converting enzyme reveals a requirement for glutamate and histidine residues. J. Biol. Chem. 281 (2006) 4596-4605. [PMID: 16361710]