Reaction: biocytin + H2O = biotin + L-lysine
Glossary: biocytin = ε-N-biotinyl-L-lysine
Other name(s): amidohydrolase biotinidase; biocytinase; biotin-amide amidohydrolase
Systematic name: biocytin amidohydrolase
Comments: The enzyme, found in many bacterial species as well as animals, liberates biotin from biocytin and short biotinylated peptides, but not from biotinylated proteins. It also has activity on biotin esters and biotin amides.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number: 9025-15-4
References:
1. Thoma, R.W. and Peterson, W.H. The enzymatic degradation of soluble bound biotin. J. Biol. Chem. 210 (1954) 569-579. [PMID: 13211594]
2. Knappe, J., Brümer, W. and Biederbick, K. Reinigung und Eigenschaften der Biotinidase aus Schweinenieren und Lactobacillus casei. Biochem. Z. 338 (1963) 599-613. [PMID: 14087327]
3. Pispa, J. and Koivusalo, M. Actinomycin D-sensitive increase in the biotinidase activity in mouse liver and serum after ethionine feeding. Acta Chem. Scand. 26 (1972) 2133-2135. [PMID: 5081874]