Reaction: (1) [lysine-biosynthesis-protein LysW]-C-terminal-γ-(L-ornithyl)-L-glutamate + H2O = [lysine-biosynthesis-protein LysW]-C-terminal-L-glutamate + L-ornithine
(2) [lysine-biosynthesis-protein LysW]-C-terminal-γ-(L-lysyl)-L-glutamate + H2O = [lysine-biosynthesis-protein LysW]-C-terminal-L-glutamate + L-lysine
Other name(s): lysK (gene name)
Systematic name: [lysine-biosynthesis-protein LysW]-C-terminal-γ-(L-ornithyl/L-lysyl)-L-glutamate amidohydrolase
Comments: The enzyme participates in an L-lysine biosynthetic pathways in certain species of archaea and bacteria. In some organisms the enzyme also catalyses the activity of EC 3.5.1.132, [amino group carrier protein]-ornithine hydrolase.
Links to other databases: BRENDA, EXPASY, ExplorEnz, KEGG, MetaCyc, PDB, CAS registry number:
References:
1. Horie, A., Tomita, T., Saiki, A., Kono, H., Taka, H., Mineki, R., Fujimura, T., Nishiyama, C., Kuzuyama, T. and Nishiyama, M. Discovery of proteinaceous N-modification in lysine biosynthesis of Thermus thermophilus. Nat. Chem. Biol. 5 (2009) 673-679. [PMID: 19620981]
2. Ouchi, T., Tomita, T., Horie, A., Yoshida, A., Takahashi, K., Nishida, H., Lassak, K., Taka, H., Mineki, R., Fujimura, T., Kosono, S., Nishiyama, C., Masui, R., Kuramitsu, S., Albers, S.V., Kuzuyama, T. and Nishiyama, M. Lysine and arginine biosyntheses mediated by a common carrier protein in Sulfolobus. Nat. Chem. Biol. 9 (2013) 277-283. [PMID: 23434852]
3. Yoshida, A., Tomita, T., Atomi, H., Kuzuyama, T. and Nishiyama, M. Lysine biosynthesis of Thermococcus kodakarensis with the capacity to function as an ornithine biosynthetic system. J. Biol. Chem. 291 (2016) 21630-21643. [PMID: 27566549]
4. Fujita, S., Cho, S.H., Yoshida, A., Hasebe, F., Tomita, T., Kuzuyama, T. and Nishiyama, M. Crystal structure of LysK, an enzyme catalyzing the last step of lysine biosynthesis in Thermus thermophilus, in complex with lysine: insight into the mechanism for recognition of the amino-group carrier protein, LysW. Biochem. Biophys. Res. Commun. 491 (2017) 409-415. [PMID: 28720495]