IUBMB Enzyme Nomenclature


Accepted name: N-isopropylammelide isopropylaminohydrolase

Reaction: N-isopropylammelide + H2O = cyanuric acid + isopropylamine

For diagram of reaction click here.

Glossary: N-isopropylammelide = 2,4-dihydroxy-6-(isopropylamino)-1,3,5-triazine
cyanuric acid = 1,3,5-triazine-2,4,6(1H,3H,5H)-trione = 2,4,6-trihydroxy-s-triazine

Other name(s): atzC (gene name)

Systematic name: N-isopropylammelide isopropylaminohydrolase

Comments: Requires Zn2+. This bacterial enzyme is involved indegradation of the herbicide atrazine. It can hydrolyse other N-substituted amino dihydroxy-s-triazine molecules, and prefers substrates with linear N-alkyl groups to those with branched alkyl groups.

Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:


1. Sadowsky, M.J., Tong, Z., de Souza, M. and Wackett, L.P. AtzC is a new member of the amidohydrolase protein superfamily and is homologous to other atrazine-metabolizing enzymes. J. Bacteriol. 180 (1998) 152-158. [PMID: 9422605]

2. Shapir, N., Osborne, J.P., Johnson, G., Sadowsky, M.J. and Wackett, L.P. Purification, substrate range, and metal center of AtzC: the N-isopropylammelide aminohydrolase involved in bacterial atrazine metabolism. J. Bacteriol. 184 (2002) 5376-5384. [PMID: 12218024]

3. Balotra, S., Warden, A.C., Newman, J., Briggs, L.J., Scott, C. and Peat, T.S. X-ray structure and mutagenesis studies of the N-isopropylammelide isopropylaminohydrolase, AtzC. PLoS One 10 (2015) e0137700. [PMID: 26390431]

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