Reaction: hydroxyatrazine + H2O = N-isopropylammelide + ethylamine
For diagram of reaction click here.
Glossary: hydroxyatrazine = 4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine
N-isopropylammelide = 2,4-dihydroxy-6-(isopropylamino)-1,3,5-triazine
Other name(s): atzB (gene name); 2,4-dihydroxy-6-(isopropylamino)-1,3,5-triazine ethylaminohydrolase
Systematic name: hydroxyatrazine ethylaminohydrolase
Comments: Contains Zn2+. This bacterial enzyme is involved indegradation of the herbicide atrazine. The enzyme has a broad substrate range, and requires a monohydroxylated s-triazine ring with a minimum of one primary or secondary amine substituent and either a chloride or amine leaving group. It catalyses both deamination and dechlorination reactions.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
References:
1. Boundy-Mills, K.L., de Souza, M.L., Mandelbaum, R.T., Wackett, L.P. and Sadowsky, M.J. The atzB gene of Pseudomonas sp. strain ADP encodes the second enzyme of a novel atrazine degradation pathway. Appl. Environ. Microbiol. 63 (1997) 916-923. [PMID: 9055410]
2. Seffernick, J.L., Aleem, A., Osborne, J.P., Johnson, G., Sadowsky, M.J. and Wackett, L.P. Hydroxyatrazine N-ethylaminohydrolase (AtzB): an amidohydrolase superfamily enzyme catalyzing deamination and dechlorination. J. Bacteriol. 189 (2007) 6989-6997. [PMID: 17660279]