IUBMB Enzyme Nomenclature


Accepted name: trans-o-hydroxybenzylidenepyruvate hydratase-aldolase

Reaction: (3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate + H2O = salicylaldehyde + pyruvate

For diagram of reaction, click here

Glossary: (3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate = (E)-2'-hydroxybenzylidenepyruvate

salicylaldehyde = 2-hydroxybenzaldehyde

Other name(s): 2'-hydroxybenzalpyruvate aldolase; NsaE; tHBPA hydratase-aldolase

Systematic name: (3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate hydro-lyase

Comments: This enzyme is involved in naphthalene degradation. The enzyme catalyses a retro-aldol reaction in vitro, and it accepts a broad range of aldehydes and 4-substituted 2-oxobut-3-enoates as substrates [4].

Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number:


1. Kuhm, A.E., Knackmuss, H.J. and Stolz, A. Purification and properties of 2'-hydroxybenzalpyruvate aldolase from a bacterium that degrades naphthalenesulfonates. J. Biol. Chem. 268 (1993) 9484-9489. [PMID: 8486638]

2. Keck, A., Conradt, D., Mahler, A., Stolz, A., Mattes, R. and Klein, J. Identification and functional analysis of the genes for naphthalenesulfonate catabolism by Sphingomonas xenophaga BN6. Microbiology 152 (2006) 1929-1940. [PMID: 16804169]

3. Eaton, R.W. Organization and evolution of naphthalene catabolic pathways: sequence of the DNA encoding 2-hydroxychromene-2-carboxylate isomerase and trans-o-hydroxybenzylidenepyruvate hydratase-aldolase from the NAH7 plasmid. J. Bacteriol. 176 (1994) 7757-7762. [PMID: 8002605]

4. Eaton, R.W. trans-o-Hydroxybenzylidenepyruvate hydratase-aldolase as a biocatalyst. Appl. Environ. Microbiol. 66 (2000) 2668-2672. [PMID: 10831455]

[EC created 2010, modified 2011]

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