Reaction: (3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate + H2O = salicylaldehyde + pyruvate
For diagram of reaction, click here
Glossary: (3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate = (E)-2'-hydroxybenzylidenepyruvate
salicylaldehyde = 2-hydroxybenzaldehyde
Other name(s): 2'-hydroxybenzalpyruvate aldolase; NsaE; tHBPA hydratase-aldolase
Systematic name: (3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate hydro-lyase
Comments: This enzyme is involved in naphthalene degradation. The enzyme catalyses a retro-aldol reaction in vitro, and it accepts a broad range of aldehydes and 4-substituted 2-oxobut-3-enoates as substrates [4].
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number:
References:
1. Kuhm, A.E., Knackmuss, H.J. and Stolz, A. Purification and properties of 2'-hydroxybenzalpyruvate aldolase from a bacterium that degrades naphthalenesulfonates. J. Biol. Chem. 268 (1993) 9484-9489. [PMID: 8486638]
2. Keck, A., Conradt, D., Mahler, A., Stolz, A., Mattes, R. and Klein, J. Identification and functional analysis of the genes for naphthalenesulfonate catabolism by Sphingomonas xenophaga BN6. Microbiology 152 (2006) 1929-1940. [PMID: 16804169]
3. Eaton, R.W. Organization and evolution of naphthalene catabolic pathways: sequence of the DNA encoding 2-hydroxychromene-2-carboxylate isomerase and trans-o-hydroxybenzylidenepyruvate hydratase-aldolase from the NAH7 plasmid. J. Bacteriol. 176 (1994) 7757-7762. [PMID: 8002605]
4. Eaton, R.W. trans-o-Hydroxybenzylidenepyruvate hydratase-aldolase as a biocatalyst. Appl. Environ. Microbiol. 66 (2000) 2668-2672. [PMID: 10831455]