Reaction: cyclobutadipyrimidine (in DNA) = 2 pyrimidine residues (in DNA)
For diagram click here.
Other name(s): photoreactivating enzyme; DNA photolyase; DNA-photoreactivating enzyme; DNA cyclobutane dipyrimidine photolyase; DNA photolyase; deoxyribonucleic photolyase; deoxyribodipyrimidine photolyase; photolyase; PRE; PhrB photolyase; deoxyribonucleic cyclobutane dipyrimidine photolyase; phr A photolyase; dipyrimidine photolyase (photosensitive); deoxyribonucleate pyrimidine dimer lyase (photosensitive)
Systematic name: deoxyribocyclobutadipyrimidine pyrimidine-lyase
Comments: A flavoprotein (FAD), containing a second chromophore group. The enzyme catalyses the reactivation by light of irradiated DNA. A similar reactivation of irradiated RNA is probably due to a separate enzyme.
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number: 37290-70-3
References:
1. Eker, A.P.M. and Fichtinger-Schepman, A.M.J. Studies on a DNA photoreactivating enzyme from Streptomyces griseus. II. Purification of the enzyme. Biochim. Biophys. Acta 378 (1975) 54-63. [PMID: 804322]
2. Sancar, G.B., Smith, F.W., Reid, R., Payne, G., Levy, M. and Sancar, A. Action mechanism of Escherichia coli DNA photolyase. I. Formation of the enzyme-substrate complex. J. Biol. Chem. 262 (1987) 478-485. [PMID: 3539939]
3. Setlow, J.K. and Bollum, F.J. The minimum size of the substrate for yeast photoreactivating enzyme. Biochim. Biophys. Acta 157 (1968) 233-237. [Medline UI: 5649902]