IUBMB Enzyme Nomenclature


Accepted name: difructose-dianhydride-III synthase

Reaction: inulobiose = α-D-fructofuranose-β-D-fructofuranose 2',1:2,3'-dianhydride + H2O

Glossary: difructose anhydride III = α-D-fructofuranose-β-D-fructofuranose 2',1:2,3'-dianhydride
inulobiose = β-D-fructofuranosyl-(2→1)-D-fructose

Other name(s): DFA-IIIase; difructose anhydride III hydrolase

Systematic name: inulobiose hydro-lyase (α-D-fructofuranose-β-D-fructofuranose 2',1:2,3'-dianhydride-forming)

Comments: The enzyme participates in an inulin degradation pathway, in which it forms inulobiose from difructose anhydride III. A conformational change in the enzyme from the bacterium Pseudarthrobacter chlorophenolicus results in it also catalysing the activity of EC, inulin fructotransferase (DFA-III-forming).

Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:


1. Tanaka, T., Uchiyama, T., Kobori, H. and Tanaka, K. Enzymic hydrolysis of di-D-fructofuranose 1, 2'; 2, 3' dianhydride with Arthrobacter ureafaciens, J. Biochem. 78 (1975) 1201-1206. [PMID: 1225919]

2. Neubauer, A., Walter, M., and Buchholz, K. Formation of inulobiose from difructoseanhydride III catalysed by a lysate from Arthrobacter ureafaciens ATCC 21124. Biocatalysis and Biotransformation 18 (2000) 443-455.

3. Saito, K., Sumita, Y., Nagasaka, Y., Tomita, F. and Yokota, A. Molecular cloning of the gene encoding the di-D-fructofuranose 1,2':2,3' dianhydride hydrolysis enzyme (DFA IIIase) from Arthrobacter sp. H65-7. J. Biosci. Bioeng. 95 (2003) 538-540. [PMID: 16233453]

4. Yu, S., Wang, X., Zhang, T., Stressler, T., Fischer, L., Jiang, B. and Mu, W. Identification of a novel di-D-fructofuranose 1,2':2,3' dianhydride (DFA III) hydrolysis enzyme from Arthrobacter aurescens SK8.001. PLoS One 10 (2015) e0142640. [PMID: 26555784]

5. Yu, S., Shen, H., Cheng, Y., Zhu, Y., Li, X., and Mu, W. Structural and functional basis of difructose anhydride III hydrolase, which sequentially converts inulin using the same catalytic residue. ACS Catalysis 8 (2018) 10683-10697.

[EC created 2021]

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