Reaction: inulobiose = bis-D-fructose 2',1:2,1'-dianhydride + H2O
Glossary: α-D-fructofuranose-β-D-fructofuranose 2',1:2,1'-dianhydride = bis-D-fructose 2',1:2,1'-dianhydride = difructose anhydride I = DFA-I
Other name(s): DFAIase; inulobiose hydrolase; bis-D-fructose 2',1:2,1'-dianhydride fructohydrolase
Systematic name: inulobiose hydro-lyase (α-D-fructofuranose-β-D-fructofuranose 2',1:2,1'-dianhydride-forming)
Comments: The enzyme, studied in the fungus Aspergillus fumigatus, may participate in an inulin degradation pathway in which it converts the product of EC 4.2.2.17, inulin fructotransferase (DFA-I-forming), to inulobiose, though in vitro activity was higher in the direction of DFA-I formation.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
References:
1. Matsuyama, T. and Tanaka, K. On the enzyme of Aspergillus fumigatus producing difructose anhydride I from inulobiose. Agric. Biol. Chem. 53 (1989) 831-832.
2. Matsuyama, T., Tanaka, K., Mashiko, M. and Kanamoto, M. Enzymic formation of di-D-fructose 1,2'; 2,1' dianhydride from inulobiose by Aspergillus fumigatus, J. Biochem. (Tokyo) 92 (1982) 1325-1328. [PMID: 6757245]