Reaction: An aliphatic amide = a nitrile + H2O
Other name(s): nitrilase; 3-cyanopyridine hydratase; NHase; L-NHase; H-NHase; acrylonitrile hydratase; aliphatic nitrile hydratase; nitrile hydro-lyase
Systematic name: aliphatic-amide hydro-lyase (nitrile-forming)
Comments: Acts on short-chain aliphatic nitriles, converting them into the corresponding acid amides. Does not act on these amides or on aromatic nitriles. cf. EC 3.5.5.1 nitrilase.
Links to other databases: BRENDA, EAWAG-BBD, EXPASY, KEGG, Metacyc, PDB, CAS registry number: 82391-37-5
References:
1. Asano, Y., Fujishiro, K., Tani, Y. and Yamada, H. Microbial degradation of nitrile compounds. 5. Aliphatic nitrile hydratase from Arthrobacter sp J-1. Purification and characterization. Agric. Biol. Chem. 46 (1982) 1165-1174.