IUBMB Enzyme Nomenclature


Accepted name: L-piperazate synthase

Reaction: N5-hydroxy-L-ornithine = (3S)-1,2-diazinane-3-carboxylate + H2O

Glossary: (3S)-1,2-diazinane-3-carboxylate = (3S)-pyridazin-3-carboxylate = L-piperazate

Other name(s): ktzT (gene name)

Systematic name: (3S)-1,2-diazinane-3-carboxylate hydrolase (N5-hydroxy-L-ornithine-forming)

Comments: Contains a heme b cofactor. The enzyme, characterized from the bacterium Kutzneria sp. 744, is one of very few enzymes known to result in the formation of an N-N bond. (3S)-1,2-diazinane-3-carboxylate (piperazate) is known to be incorporated into assorted secondary products that are produced by nonribosomal peptide synthetase or nonribosomal peptide synthetase/polyketide synthase hybrid pathways, such as the kutznerides, padanamides, himastatins, and sanglifehrins.

Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:


1. Du, Y.L., He, H.Y., Higgins, M.A. and Ryan, K.S. A heme-dependent enzyme forms the nitrogen-nitrogen bond in piperazate. Nat. Chem. Biol. 13 (2017) 836-838. [PMID: 28628093]

[EC created 2021]

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