IUBMB Enzyme Nomenclature


Accepted name: aliphatic aldoxime dehydratase

Reaction: an aliphatic aldoxime = an aliphatic nitrile + H2O

Other name(s): OxdA; aliphatic aldoxime hydro-lyase

Systematic name: aliphatic aldoxime hydro-lyase (aliphatic-nitrile-forming)

Comments: The enzyme from Pseudomonas chlororaphis contains Ca2+ and protoheme IX, the iron of which must be in the form iron(II) for activity. The enzyme exhibits a strong preference for aliphatic aldoximes, such as butyraldoxime and acetaldoxime, over aromatic aldoximes, such as pyridine-2-aldoxime, which is a poor substrate. No activity was found with the aromatic aldoximes benzaldoxime and pyridine-4-aldoxime.

Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:


1. Oinuma, K.-I., Hashimoto, Y., Konishi, K., Goda, M., Noguchi, T., Higashibata, H. and Kobayashi, M. Novel aldoxime dehydratase involved in carbon-nitrogen triple bond synthesis of Pseudomonas chlororaphis B23: Sequencing, gene expression, purification and characterization. J. Biol. Chem. 278 (2003) 29600-29608. [PMID: 12773527]

2. Xie, S.X., Kato, Y., Komeda, H., Yoshida, S. and Asano, Y. A gene cluster responsible for alkylaldoxime metabolism coexisting with nitrile hydratase and amidase in Rhodococcus globerulus A-4. Biochemistry 42 (2003) 12056-12066. [PMID: 14556637]

3. Kato, Y., Yoshida, S., Xie, S.-X. and Asano, Y. Aldoxime dehydratase co-existing with nitrile hydratase and amidase in the iron-type nitrile hydratase-producer Rhodococcus sp. N-771. J. Biosci. Bioeng. 97 (2004) 250-259. [PMID: 16233624]

[EC created 2004 as EC, transferred 2021 to EC]

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