Reaction: an N-(sulfonatooxy)alkanimidothioate = a nitrile + sulfate + sulfur
Glossary: an N-(sulfonatooxy)alkanimidothioate = a thiohydroximate-O-sulfate
Other name(s): NSP (gene name); nitrile-specifier protein
Systematic name: thiohydroximate-O-sulfate sulfate/sulfur-lyase (nitrile-forming)
Comments: The enzyme is involved in the breakdown of glucosinolates. It can act on both aliphatic and aromatic glucosinolates, and forms nitrile-containing products. cf. EC 4.8.1.6, N-(sulfonatooxy)alkenimidothioic acid sulfate-lyase (epithionitrile-forming), and EC 4.8.1.7, phenyl-N-(sulfonatooxy)methanimidothioate sulfolyase.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
References:
1. Kissen, R. and Bones, A.M. Nitrile-specifier proteins involved in glucosinolate hydrolysis in Arabidopsis thaliana. J. Biol. Chem. 284 (2009) 12057-12070. [PMID: 19224919]
2. Burow, M., Losansky, A., Muller, R., Plock, A., Kliebenstein, D.J. and Wittstock, U. The genetic basis of constitutive and herbivore-induced ESP-independent nitrile formation in Arabidopsis. Plant Physiol. 149 (2009) 561-574. [PMID: 18987211]