Reaction: UDP-N-acetyl-α-D-glucosamine = UDP-N-acetyl-α-D-mannosamine
For diagram of reaction click here
Other name(s): UDP-N-acetylglucosamine 2'-epimerase (ambiguous); uridine diphosphoacetylglucosamine 2'-epimerase (ambiguous); uridine diphospho-N-acetylglucosamine 2'-epimerase (ambiguous); uridine diphosphate-N-acetylglucosamine-2'-epimerase (ambiguous); rffE (gene name); mnaA (gene name); UDP-N-acetyl-D-glucosamine 2-epimerase
Systematic name: UDP-N-acetyl-α-D-glucosamine 2-epimerase
Comments: This bacterial enzyme catalyses the reversible interconversion of UDP-GlcNAc and UDP-ManNAc. The latter is used in a variety of bacterial polysaccharide biosyntheses. cf. EC 3.2.1.183, UDP-N-acetylglucosamine 2-epimerase (hydrolysing).
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number: 9037-71-2
References:
1. Kawamura, T., Kimura, M., Yamamori, S. and Ito, E. Enzymatic formation of uridine diphosphate N-acetyl-D-mannosamine. J. Biol. Chem. 253 (1978) 3595-3601. [PMID: 418068]
2. Meier-Dieter, U., Starman, R., Barr, K., Mayer, H. and Rick, P.D. Biosynthesis of enterobacterial common antigen in Escherichia coli. Biochemical characterization of Tn10 insertion mutants defective in enterobacterial common antigen synthesis. J. Biol. Chem. 265 (1990) 13490-13497. [PMID: 2166030]
3. Morgan, P. M., Sala, R. F., and Tanner, M. E. Eliminations in the reactions catalyzed by UDP-N-acetylglucosamine 2-epimerase. J. Am. Chem. Soc. 119 (1997) 10269-10277.
4. Campbell, R.E., Mosimann, S.C., Tanner, M.E. and Strynadka, N.C. The structure of UDP-N-acetylglucosamine 2-epimerase reveals homology to phosphoglycosyl transferases. Biochemistry 39 (2000) 14993-15001. [PMID: 11106477]
5. Samuel, J. and Tanner, M.E. Active site mutants of the "non-hydrolyzing" UDP-N-acetylglucosamine 2-epimerase from Escherichia coli. Biochim. Biophys. Acta 1700 (2004) 85-91. [PMID: 15210128]
6. Soldo, B., Lazarevic, V., Pooley, H.M. and Karamata, D. Characterization of a Bacillus subtilis thermosensitive teichoic acid-deficient mutant: gene mnaA (yvyH) encodes the UDP-N-acetylglucosamine 2-epimerase. J. Bacteriol. 184 (2002) 4316-4320. [PMID: 12107153]