EC 6.1.1.10

Reaction: ATP + L-methionine + tRNA^Met = AMP + diphosphate + L-methionyl-tRNA^Met

Other name(s): methionyl-tRNA synthetase; methionyl-transfer ribonucleic acid synthetase; methionyl-transfer ribonucleate synthetase; methionyl-transfer RNA synthetase; methionine translase; MetRS

Systematic name: L-methionine:tRNA^Met ligase (AMP-forming)

Comments: In those organisms producing N-formylmethionyl-tRNA^fMet for translation initiation, this enzyme also recognizes the initiator tRNA^fMet and catalyses the formation of L-methionyl-tRNA^fMet, the substrate for EC 2.1.2.9, methionyl-tRNA formyltransferase.

Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number: 9033-22-1

References:

1. Bergmann, F.H., Berg, P. and Dieckmann, M. The enzymic synthesis of amino acyl derivatives of ribonucleic acid. II. The preparation of leucyl-, valyl-, isoleucyl- and methionyl ribonucleic acid synthetases from Escherichia coli. J. Biol. Chem. 236 (1961) 1735-1740.

2. Lee, C.P., Dyson, M.R., Mandal, N., Varshney, U., Bahramian, B. and RajBhandary, U.L. Striking effects of coupling mutations in the acceptor stem on recognition of tRNAs by Escherichia coli Met-tRNA synthetase and Met-tRNA transformylase. 89 (1992) 9262-9266. [PMID: 1409632]