IUBMB Enzyme Nomenclature


Accepted name: 3-oxocholest-4-en-26-oate—CoA ligase

Reaction: ATP + (25S)-3-oxocholest-4-en-26-oate + CoA = AMP + diphosphate + (25S)-3-oxocholest-4-en-26-oyl-CoA

For diagram of reaction click here.

Other name(s): fadD19 (gene name)

Systematic name: (25S)-3-oxocholest-4-en-26-oate:CoA ligase (AMP-forming)

Comments: The enzyme, characterized from actinobacterium Mycobacterium tuberculosis, catalyses a step in the degradation of cholesterol. It is responsible for the activation of the C8 side chain. 3β-hydroxycholest-5-en-26-oate can also be used as substrate.

Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number:


1. Wilbrink, M.H., Petrusma, M., Dijkhuizen, L. and van der Geize, R. FadD19 of Rhodococcus rhodochrous-DSM43269, a steroid-coenzyme A ligase essential for degradation of C-24 branched sterol side chains. Appl. Environ. Microbiol. 77 (2011) 4455-4464. [PMID: 21602385]

2. Casabon, I., Swain, K., Crowe, A.M., Eltis, L.D. and Mohn, W.W. Actinobacterial acyl coenzyme a synthetases involved in steroid side-chain catabolism. J. Bacteriol. 196 (2014) 579-587. [PMID: 24244004]

[EC created 2014]

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