IUBMB Enzyme Nomenclature


Accepted name: malonate—CoA ligase

Reaction: ATP + malonate + CoA = AMP + phosphate + malonyl-CoA

Other name(s): ACSF3 (gene name); AAE13 (gene name); malonyl-CoA synthetase

Systematic name: malonate:CoA ligase (AMP-forming)

Comments: The enzyme, found in mitochondria, detoxifies malonate, which is a potent inhibitor of mitochondrial respiration, and provides malonyl-CoA to the mitochondrial fatty acid biosynthesis pathway.

Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number:


1. Gueguen, V., Macherel, D., Jaquinod, M., Douce, R. and Bourguignon, J. Fatty acid and lipoic acid biosynthesis in higher plant mitochondria. J. Biol. Chem. 275 (2000) 5016-5025. [PMID: 10671542]

2. Witkowski, A., Thweatt, J. and Smith, S. Mammalian ACSF3 protein is a malonyl-CoA synthetase that supplies the chain extender units for mitochondrial fatty acid synthesis. J. Biol. Chem. 286 (2011) 33729-33736. [PMID: 21846720]

3. Chen, H., Kim, H.U., Weng, H. and Browse, J. Malonyl-CoA synthetase, encoded by Acyl Activating Enzyme13, is essential for growth and development of Arabidopsis. Plant Cell 23 (2011) 2247-2262. [PMID: 21642549]

4. Guan, X. and Nikolau, B.J. AAE13 encodes a dual-localized malonyl-CoA synthetase that is crucial for mitochondrial fatty acid biosynthesis. Plant J. 85 (2016) 581-593. [PMID: 26836315]

5. Bowman, C.E., Rodriguez, S., Selen Alpergin, E.S., Acoba, M.G., Zhao, L., Hartung, T., Claypool, S.M., Watkins, P.A. and Wolfgang, M.J. The mammalian malonyl-CoA synthetase ACSF3 is required for mitochondrial protein malonylation and metabolic efficiency. Cell Chem. Biol. 24 (2017) 673-684.e4. [PMID: 28479296]

6. Bowman, C.E. and Wolfgang, M.J. Role of the malonyl-CoA synthetase ACSF3 in mitochondrial metabolism. Adv Biol Regul 71 (2019) 34-40. [PMID: 30201289]

[EC created 2022]

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