Reaction: ATP + L-lysine + [L-lysyl-carrier protein] = AMP + diphosphate + L-lysyl-[L-lysyl-carrier protein] (overall reaction)
(1a) ATP + L-lysine = diphosphate + (L-lysyl)adenylate
(1b) (L-lysyl)adenylate + [L-lysyl-carrier protein] = AMP + L-lysyl-[L-lysyl-carrier protein]
Other name(s): scoA (gene name)
Systematic name: L-lysine:[L-lysyl-carrier protein] ligase (AMP-forming)
Comments: The adenylation domain of the enzyme catalyses the activation of L-lysine to (L-lysyl)adenylate, followed by the transfer of the activated compound to the free thiol of a phosphopantetheine arm of a peptidyl-carrier protein domain. The peptidyl-carrier protein domain may be part of the same protein (as in the case of ScoA), or of a different protein. This activity is often found as part of a larger non-ribosomal peptide synthase.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
References:
1. Harris, N.C., Sato, M., Herman, N.A., Twigg, F., Cai, W., Liu, J., Zhu, X., Downey, J., Khalaf, R., Martin, J., Koshino, H. and Zhang, W. Biosynthesis of isonitrile lipopeptides by conserved nonribosomal peptide synthetase gene clusters in Actinobacteria. Proc. Natl. Acad. Sci. USA 114 (2017) 7025-7030. [PMID: 28634299]