Reaction: 2 ATP + 2 3-phenyl-2-oxopropanoate = 2 AMP + 2 diphosphate + polyporic acid (overall reaction)
(1a) ATP + 3-phenyl-2-oxopropanoate + [polyporic acid synthase] = AMP + diphosphate + 3-pheny-2-oxopropanoyl-[polyporic acid synthase]
(1b) ATP + 3-phenyl-2-oxopropanoate + 3-pheny-2-oxopropanoyl-[polyporic acid synthase] = AMP + diphosphate + polyporic acid + [polyporic acid synthase]
Glossary: polyporic acid = 2,5-dihydroxy-3,6-diphenylcyclohexa-2,5-diene-1,4-dione
3-phenyl-2-oxopropanoate = 3-phenylpyruvate
Other name(s): polyporic acid synthetase; corA (gene name); hapA1 (gene name); hapA2 (gene name)
Systematic name: 3-phenyl-2-oxopropanoate:3-phenyl-2-oxopropanoate ligase (polyporic acid-forming)
Comments: Requires Mg2+. The enzyme, found in mushrooms of the Basidiomycota and Ascomycota phyla, is a non-ribosomal peptide synthase-like enzyme comprising three domains - an adelylation (A) domain, an ayl-carrier protein (ACP) domain, and a thioesterase (TE) domain. The enzyme activates 3-phenyl-2-oxopropanoate by adenylation and catalyses the dimerization/cyclization of two such molecules to form polyporic acid. Polyporic acid is a precursor for assorted metabolites produced by the fungi, such as the corticins.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
References:
1. Lawrinowitz, S., Wurlitzer, J.M., Weiss, D., Arndt, H.D., Kothe, E., Gressler, M. and Hoffmeister, D. Blue light-dependent pre-mRNA splicing controls pigment biosynthesis in the mushroom Terana caerulea. Microbiol. Spectr. 10 (2022) e0106522. [PMID: 36094086]
2. Seibold, P.S., Lawrinowitz, S., Raztsou, I., Gressler, M., Arndt, H.D., Stallforth, P. and Hoffmeister, D. Bifurcate evolution of quinone synthetases in basidiomycetes. Fungal Biol Biotechnol 10 (2023) 14. [PMID: 37400920]