EC 6.4.1.6

Reaction: acetone + hydrogen carbonate + ATP + 2 H₂O = acetoacetate + AMP + 2 phosphate

Systematic name: acetone:carbon-dioxide ligase (AMP-forming)

Comments: Requires Mg²⁺ and ATP. The enzyme from Xanthobacter sp. strain Py2 also carboxylates butan-2-one to 3-oxopentanoate.

Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number: 189258-15-9

References:

1. Sluis, M.K. and Ensign, S.A. Purification and characterization of acetone carboxylase from Xanthobacter strain Py2. Proc. Natl. Acad. Sci. USA 94 (1997) 8456-8461. [PMID: 9237998]

2. Schuhle, K. and Heider, J. Acetone and butanone metabolism of the denitrifying bacterium Aromatoleum aromaticum demonstrates novel biochemical properties of an ATP-dependent aliphatic ketone carboxylase. J. Bacteriol. 194 (2012) 131–141. [PMID: 22020645]