Reaction: ATP + 3-amino-2-hydroxy-4-methoxybenzoate + nitrite = AMP + diphosphate + cremeomycin + H2O
For diagram of reaction, click here
Glossary: cremeomycin = 6-carboxy-2-diazonio-3-methoxyphenolate
Other name(s): creM (gene name)
Systematic name: 3-amino-2-hydroxy-4-methoxybenzoate:nitrite ligase (AMP-forming)
Comments: The enzyme, characterized from Streptomyces cremeus, catalyses the last step in the biosynthesis of the ortho-diazoquinone cremeomycin.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
References:
1. Waldman, A.J. and Balskus, E.P. Discovery of a diazo-forming enzyme in cremeomycin biosynthesis. J. Org. Chem. 83 (2018) 7539-7546. [PMID: 29771512]