IUBMB Enzyme Nomenclature


Accepted name: ferredoxin—NAD+ oxidoreductase (H+-transporting)

Reaction: 2 reduced ferredoxin [iron-sulfur] cluster + NAD+ + H+ + 2 H+[side 1] = 2 oxidized ferredoxin [iron-sulfur] cluster + NADH + 2 H+[side 2]

Other name(s): Rnf complex (ambiguous); H+-translocating ferredoxin:NAD+ oxidoreductase

Systematic name: ferredoxin:NAD+ oxidoreductase (H+-transporting)

Comments: This iron-sulfur and flavin-containing electron transport complex, isolated from some anaerobic bacteria, couples the energy from reduction of NAD+ by ferredoxin to pumping protons out of the cell, generating a proton motive force across the cytoplasmic membrane. Most similar complexes pump sodium ions rather than protons [cf. EC, ferredoxin—NAD+ oxidoreductase (Na+-transporting)].

Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:


1. Tremblay, P.L., Zhang, T., Dar, S.A., Leang, C. and Lovley, D.R. The Rnf complex of Clostridium ljungdahlii is a proton-translocating ferredoxin:NAD+ oxidoreductase essential for autotrophic growth. mBio 4 (2012) e00406. [PMID: 23269825]

2. Wang, L., Bradstock, P., Li, C., McInerney, M.J. and Krumholz, L.R. The role of Rnf in ion gradient formation in Desulfovibrio alaskensis. PeerJ 4 (2016) e1919. [PMID: 27114876]

[EC created 2021]

Return to EC 7.1.1 home page
Return to EC 7.1 home page
Return to EC 7 home page
Return to Enzymes home page
Return to IUBMB Biochemical Nomenclature home page