The mechanism below rationalizes the reaction mechanism, see Byford, M.F., Baldwin, J.E., Shiau, C.-Y. and Schofield, C.J. The mechanism of ACV synthetase. Chem. Rev. 97 (1997) 2631-2649. [PMID: 11851475]
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penicillin biosynthesis
EC 6.3.2.26 N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase