IUBMB Enzyme Nomenclature


Accepted name: L-proline trans-4-hydroxylase

Reaction: L-proline + 2-oxoglutarate + O2 = trans-4-hydroxy-L-proline + succinate + CO2

Systematic name: L-proline,2-oxoglutarate:oxygen oxidoreductase (trans-4-hydroxylating)

Comments: Requires Fe2+ and ascorbate. The enzyme, isolated from multiple bacterial species, only produces trans-4-hydroxy-L-proline (cf. EC, L-proline cis-4-hydroxylase).

Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:


1. Lawrence, C.C., Sobey, W.J., Field, R.A., Baldwin, J.E. and Schofield, C.J. Purification and initial characterization of proline 4-hydroxylase from Streptomyces griseoviridus P8648: a 2-oxoacid, ferrous-dependent dioxygenase involved in etamycin biosynthesis. Biochem. J. 313 (1996) 185-191. [PMID: 8546682]

2. Shibasaki, T., Mori, H., Chiba, S. and Ozaki, A. Microbial proline 4-hydroxylase screening and gene cloning. Appl. Environ. Microbiol. 65 (1999) 4028-4031. [PMID: 10473412]

[EC created 2017]

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