Reaction: β-colubrine + [reduced NADPH—hemoprotein reductase] + O2 = 11-demethylbrucine + [oxidized NADPH—hemoprotein reductase] + H2O
For diagram of reaction click here
Glossary: β-colubrine = 2-methoxystrychnidine (IUPAC) = 10-methoxystrychnidine (ref 1)
11-demethylbrucine (ref 1) = 3-hydroxy-2-methoxystrichnidine (IUPAC)
Other name(s): CYP71AH44
Systematic name: β-colubrine,[NADPH—hemoprotein reductase]:oxygen oxidoreductase (11-hydroxylating)
Comments: A cytochrome P-450 (heme-thiolate) protein. The enzyme, isolated from the tree Strychnos nux-vomica, participates in the biosynthesis of brucine.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
References:
1. Hong, B., Grzech, D., Caputi, L., Sonawane, P., Lopez, C.ER., Kamileen, M.O., Hernandez Lozada, N.J., Grabe, V. and O'Connor, S.E. Biosynthesis of strychnine. Nature 607 (2022) 617-622. [PMID: 35794473]