IUBMB Enzyme Nomenclature


Accepted name: dihydrofolate reductase

Reaction: 5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH + H+

For diagram of reaction click here.

Other name(s): tetrahydrofolate dehydrogenase; DHFR; pteridine reductase:dihydrofolate reductase; dihydrofolate reductase:thymidylate synthase; thymidylate synthetase-dihydrofolate reductase; folic acid reductase; folic reductase; dihydrofolic acid reductase; dihydrofolic reductase; 7,8-dihydrofolate reductase; NADPH-dihydrofolate reductase

Systematic name: 5,6,7,8-tetrahydrofolate:NADP+ oxidoreductase

Comments: The enzyme from animals and some micro-organisms also slowly reduces folate to 5,6,7,8-tetrahydrofolate.

Links to other databases: BRENDA, EXPASY, GTD, KEGG, Metacyc, PDB, CAS registry number: 9002-03-3


1. Blakley, R.L. and MacDougall, B.M. Dihydrofolic reductase from Streptococcus faecalis R. J. Biol. Chem. 236 (1961) 1163.

2. Bolin, J.T., Filman, D.J., Matthews, D.A. and Kraut, J. Crystal structures of Escherichia coli and Lactobacillus casei dihydrofolate reductase refined at 1.7 Å resolution. I. General features and binding of methotrexate. J. Biol. Chem. 257 (1982) 13650-13662. [PMID: 6815178]

3. Kaufman, B.T. and Gardiner, R.C. Studies on dihydrofolic reductase. I. Purification and properties of dihydrofolic reductase from chicken liver. J. Biol. Chem. 241 (1966) 1319-1328. [PMID: 4379915]

4. Young, I.G. and Gibson, F. Regulation of the enzymes involved in the biosynthesis of 2,3-dihydroxybenzoic acid in Aerobacter aerogenes and Escherichia coli. Biochim. Biophys. Acta 177 (1969) 401-411. [PMID: 4306838]

[EC created 1961, modified 1976 (EC created 1961, incorporated 1976)]

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