IUBMB Enzyme Nomenclature


Accepted name: methylenetetrahydrofolate reductase (ferredoxin)

Reaction: 5-methyltetrahydrofolate + 2 oxidized ferredoxin = 5,10-methylenetetrahydrofolate + 2 reduced ferredoxin + 2 H+

For diagram of reaction click here

Other name(s): 5,10-methylenetetrahydrofolate reductase

Systematic name: 5-methyltetrahydrofolate:ferredoxin oxidoreductase

Comments: An iron-sulfur flavoprotein that also contains zinc. The enzyme from Clostridium formicoaceticum catalyses the reduction of methylene blue, menadione, benzyl viologen, rubredoxin or FAD with 5-methyltetrahydrofolate and the oxidation of reduced ferredoxin or FADH2 with 5,10-methylenetetrahydrofolate. However, unlike EC, methylenetetrahydrofolate reductase (NADPH); EC, methylenetetrahydrofolate reductase (NADH); or EC, methylenetetrahydrofolate reductase [NAD(P)H], there is no activity with either NADH or NADP.

Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:


1. Clark, J.E. and Ljungdahl, L.G. Purification and properties of 5,10-methylenetetrahydrofolate reductase, an iron-sulfur flavoprotein from Clostridium formicoaceticum. J. Biol. Chem. 259 (1984) 10845-10849. [PMID: 6381490]

[EC created 2005, modified 2021]

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