IUBMB Enzyme Nomenclature


Accepted name: thymidylate synthase

Reaction: 5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP

For diagram of reaction click here.

Other name(s): dTMP synthase; thymidylate synthetase; methylenetetrahydrofolate:dUMP C-methyltransferase; TMP synthetase

Systematic name: 5,10-methylenetetrahydrofolate:dUMP C-methyltransferase

Links to other databases: BRENDA, EXPASY, GTD, KEGG, Metacyc, PDB, CAS registry number: 9031-61-2


1. Blakley, R.L. The biosynthesis of thymidylic acid. IV. Further studies on thymidylate synthase. J. Biol. Chem. 238 (1963) 2113-2118.

2. Lockshin, A., Moran, R.G. and Danenberg, P.V. Thymidylate synthetase purified to homogeneity from human leukemic cells. Proc. Natl. Acad. Sci. USA 76 (1979) 750-754. [PMID: 34155]

3. Slavik, K. and Slavikova, V. Purification of thymidylate synthetase from enzyme-poor sources by affinity chromatography. Methods Enzymol. 66 (1980) 709-723. [PMID: 6990200]

4. Wahba, A.J. and Friedkin, M. The enzymatic synthesis of thymidylate. I. Early steps in the purification of thymidylate synthetase of Escherichia coli. J. Biol. Chem. 237 (1962) 3794-3801.

[EC created 1976]

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