Reaction: L-alanine + 2-oxoglutarate = pyruvate + L-glutamate
For diagram click here (mechanism).
Other name(s): glutamic-pyruvic transaminase; glutamic-alanine transaminase; GPT (ambiguous); alanine aminotransferase; alanine-α-ketoglutarate aminotransferase; alanine-pyruvate aminotransferase; ALT; glutamic acid-pyruvic acid transaminase; glutamic-pyruvic aminotransferase; L-alanine aminotransferase; L-alanine transaminase; L-alanine-α-ketoglutarate aminotransferase; pyruvate transaminase; pyruvate-alanine aminotransferase; pyruvate-glutamate transaminase
Systematic name: L-alanine:2-oxoglutarate aminotransferase
Comments: A pyridoxal-phosphate protein. 2-Aminobutanoate can act slowly instead of alanine.
Links to other databases: BRENDA, EXPASY, GTD, KEGG, Metacyc, PDB, CAS registry number: 9000-86-6
References:
1. Dumitru, I.F., Iordachescu, D. and Niculescu, S. Chromatographic purification, crystallization and study of vegetable L-alanine: 2-oxoglutarate-aminotransferase properties. Experientia 26 (1970) 837-838. [PMID: 5452003].
2. Dumitru, I.F., Iordachescu, D. and Niculescu, S. L-Alanine: 2-oxoglutarate-aminotransferase chromatographic purification and crystallization of the enzyme from seeds of Glycine hispida var Cheepeura. Rev. Roum. Biochim. 7 (1970) 31-44.
3. Green, D.E., Leloir, L.F. and Nocito, W. Transaminases. J. Biol. Chem. 161 (1945) 559-582.
4. Iordachescu, D., Dumitru, I.F. and Corniciuc, M.-T. Comparative biochemical studies concerning L-alanine: 2-oxoglutarate-aminotransferase from the liver and the bile of swines. Rev. Roum. Biochim. 20 (1983) 173-179.
5. Wilson, D.G., King, K.W. and Burris, R.H. Transaminase reactions in plants. J. Biol. Chem. 208 (1954) 863-874.