Reaction: (1) ATP + [endosulfine family protein]-L-serine = ADP + [endosulfine family protein]-O-phospho-L-serine
(2) ATP + [endosulfine family protein]-L-threonine = ADP + [endosulfine family protein]-O-phospho-L-threonine
Glossary: MASTL = microtubule-associated
Other name(s): MASTL; gwl; greatwall kinase; RIM15; microtubule-associated (MASTL)-subfamily-protein kinase.
Systematic name: ATP:[endosulfine family protein] phosphotransferase (Ser/Thr-phosphorylating)
Comments: Requires Mg2+. Peptide array data suggest that MASTL prefers to phosphorylate Ser over Thr, followed at position +1 by a bulky hydrophobic (Met, Leu, Phe or Tyr) and with an aromatic residue in the -2 position [4]. Its main role in animal systems is in the regulation of mitosis by phosphorylating a conserved site on endosulfine family proteins (ENSA and ARPP-19 in human), causing them to inhibit the protein phosphatase 2A-B55 [protein phosphatase 2A (PP2A) holoenzyme complex containing a B55-family regulatory subunit] [2].
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
References:
1. Pearce, L.R., Komander, D. and Alessi, D.R. The nuts and bolts of AGC protein kinases. Nat. Rev. Mol. Cell. Biol. 11 (2010) 9-22. [PMID: 20027184]
2. Castro, A. and Lorca, T. Greatwall kinase at a glance. J. Cell Sci. 131 (2018) . [PMID: 30355803]
3. Goguet-Rubio, P., Amin, P., Awal, S., Vigneron, S., Charrasse, S., Mechali, F., Labbe, J.C., Lorca, T. and Castro, A. PP2A-B55 holoenzyme regulation and cancer. Biomolecules 10 (2020) . [PMID: 33266510]
4. Johnson, J.L., Yaron, T.M., Huntsman, E.M., Kerelsky, A., Song, J., Regev, A., Lin, T.Y., Liberatore, K., Cizin, D.M., Cohen, B.M., Vasan, N., Ma, Y., Krismer, K., Robles, J.T., van de Kooij, B., van Vlimmeren, A.E., Andree-Busch, N., Kaufer, N.F., Dorovkov, M.V., Ryazanov, A.G., Takagi, Y., Kastenhuber, E.R., Goncalves, M.D., Hopkins, B.D., Elemento, O., Taatjes, D.J., Maucuer, A., Yamashita, A., Degterev, A., Uduman, M., Lu, J., Landry, S.D., Zhang, B., Cossentino, I., Linding, R., Blenis, J., Hornbeck, P.V., Turk, B.E., Yaffe, M.B. and Cantley, L.C. An atlas of substrate specificities for the human serine/threonine kinome. Nature 613 (2023) 759-766. [PMID: 36631611]