Reaction: 2'-deoxyribonucleoside 5'-triphosphate + DNAn = diphosphate + DNAn+1
Other name(s): terminal deoxyribonucleotidyltransferase; terminal addition enzyme; addase; deoxynucleotidyl terminal transferase; deoxyribonucleic acid nucleotidyltransferase; deoxyribonucleic nucleotidyltransferase; terminal deoxynucleotide transferase; TdT
Systematic name: 2'-deoxyribonucleoside-5'-triphosphate:DNA deoxynucleotidylexotransferase
Comments: Catalyses template-independent extension of the 3'- end of a DNA strand by one nucleotide at a time. Cannot initiate a chain de novo. The nucleoside may be ribo- or 2'-deoxyribo-.
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number: 9027-67-2
References:
1. Bollum, F.J. Deoxynucleotide-polymerizing enzymes of calf thymus gland. V. Homogeneous terminal deoxynucleotidyl transferase. J. Biol. Chem. 246 (1971) 909-916. [PMID: 5543689]
2. Gottesman, M.E. and Canellakis, E.S. The terminal nucleotidyltransferases of calf thymus nuclei. J. Biol. Chem. 241 (1966) 4339-4352. [PMID: 4288534]
3. Krakow, J.S., Coutsogeorgopoulos, C. and Canellakis, E.S. Studies on the incorporation of deoxyribonucleic acid. Biochim. Biophys. Acta 55 (1962) 639-650.