IUBMB Enzyme Nomenclature


Accepted name: GDP polyribonucleotidyltransferase

Reaction: (5')pppAACA-[mRNA] + GDP = diphosphate + G(5')pppAACA-[mRNA] (overall reaction)
(1a) (5')pppAACA-[mRNA] + [protein L]-L-histidine = diphosphate + [protein L]-L-histidyl-(5')phosphonato-AACA-[mRNA] + H2O
(1b) [protein L]-L-histidyl-(5')phosphonato-AACA-[mRNA] + GDP + H2O = [protein L]-L-histidine + G(5')pppAACA-[mRNA]

Other name(s): PRNTase; 5'-triphospho-mRNA:GDP 5'-phosphopolyribonucleotidyltransferase [G(5')ppp-mRNA-forming]

Systematic name: (5')pppAACA-[mRNA]:GDP 5'-phosphopolyribonucleotidyltransferase [(5')pppAACA-[mRNA]-forming]

Comments: The enzyme from non-segmented negative strain (NNS) viruses (e.g. rhabdoviruses and lyssaviruses) is specific for mRNAs with sequences starting with AACA. cf. EC, mRNA guanylyltransferase.

Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number:


1. Ogino, T. and Banerjee, A.K. Unconventional mechanism of mRNA capping by the RNA-dependent RNA polymerase of vesicular stomatitis virus. Mol. Cell 25 (2007) 85-97. [PMID: 17218273]

2. Ogino, T. and Banerjee, A.K. Formation of guanosine(5')tetraphospho(5')adenosine cap structure by an unconventional mRNA capping enzyme of vesicular stomatitis virus. J. Virol. 82 (2008) 7729-7734. [PMID: 18495767]

3. Ogino, T., Yadav, S.P. and Banerjee, A.K. Histidine-mediated RNA transfer to GDP for unique mRNA capping by vesicular stomatitis virus RNA polymerase. Proc. Natl. Acad. Sci. USA 107 (2010) 3463-3468. [PMID: 20142503]

4. Ogino, T. and Banerjee, A.K. The HR motif in the RNA-dependent RNA polymerase L protein of Chandipura virus is required for unconventional mRNA-capping activity. J. Gen. Virol. 91 (2010) 1311-1314. [PMID: 20107017]

5. Ogino, T. and Banerjee, A.K. An unconventional pathway of mRNA cap formation by vesiculoviruses. Virus Res. 162 (2011) 100-109. [PMID: 21945214]

6. Ogino, M., Ito, N., Sugiyama, M. and Ogino, T. The rabies virus L protein catalyzes mRNA capping with GDP polyribonucleotidyltransferase activity. Viruses 8 (2016) 144. [PMID: 27213429]

[EC created 2015, modified 2020]

Return to EC 2.7.7 home page
Return to EC 2.7 home page
Return to EC 2 home page
Return to Enzymes home page
Return to IUBMB Biochemical Nomenclature home page