Reaction: ATP + rifampicin + H2O = AMP + 21-phosphorifampicin + phosphate
For diagram of reaction click here.
Glossary: rifampicin = rifampin = 3-[(4-methylpiperazin-1-yl)iminomethyl]rifamycin
Other name(s): rifampin phosphotransferase; RPH
Systematic name: ATP:rifampicin, water 21-O-phosphotransferase
Comments: The enzyme, characterized from a diverse collection of Gram-positive bacteria, inactivates the antibiotic rifampicin by phosphorylating it at position 21. The enzyme comprises three domains: two substrate-binding domains (ATP-grasp and rifampicin-binding domains) and a smaller phosphate-carrying L-histidine swivel domain that transits between the spatially distinct substrate-binding sites during catalysis.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
1. Spanogiannopoulos, P., Waglechner, N., Koteva, K. and Wright, G.D. A rifamycin inactivating phosphotransferase family shared by environmental and pathogenic bacteria. Proc. Natl Acad. Sci. USA 111 (2014) 7102-7107. [PMID: 24778229]
2. Stogios, P.J., Cox, G., Spanogiannopoulos, P., Pillon, M.C., Waglechner, N., Skarina, T., Koteva, K., Guarne, A., Savchenko, A. and Wright, G.D. Rifampin phosphotransferase is an unusual antibiotic resistance kinase. Nat Commun 7 (2016) 11343. [PMID: 27103605]