Reaction: Hydrolysis of internal α-D-arabinofuranoside bonds in D-arabinans
Other name(s): endo-D-arabinanase (ambiguous); DgGH4185a; DgGH4185b; MyxoGH4185; PhageGH4185; Mab4185; EndoMA1; EndoMA2.
Systematic name: D-arabinan endo α-(1,5)-arabinofuranosidase (configuration-retaining)
Comments: The enzyme hydrolyses α-(1,5)-D-arabinofuranoside bonds in D-arabinan core structure of lipoarabinomannan and arabinogalactan of mycobacterial cell wall. cf. EC 3.2.1.224, D-arabinan exo β-(1,2)-arabinofuranosidase (non-reducing end); cf. EC 3.2.1.225, D-arabinan exo α-(1,3)/(1,5)-arabinofuranosidase (non-reducing end).
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
References:
1. Kotani, S., Kato, T., Matsuda, T., Kato, K. and Misaki, A. Chemical structure of the antigenic determinants of cell wall polysaccharide of Mycobacterium tuberculosis strain H37Rv. Biken J 14 (1971) 379-387. [PMID: 4113500]
2. Al-Jourani, O., Benedict, S.T., Ross, J., Layton, A.J., van der Peet, P., Marando, V.M., Bailey, N.P., Heunis, T., Manion, J., Mensitieri, F., Franklin, A., Abellon-Ruiz, J., Oram, S.L., Parsons, L., Cartmell, A., Wright, G.SA., Basle, A., Trost, M., Henrissat, B., Munoz-Munoz, J., Hirt, R.P., Kiessling, L.L., Lovering, A.L., Williams, S.J., Lowe, E.C. and Moynihan, P.J. Identification of D-arabinan-degrading enzymes in mycobacteria. Nat. Commun. 14 (2023) 2233. [PMID: 37076525]
3. Shimokawa, M., Ishiwata, A., Kashima, T., Nakashima, C., Li, J., Fukushima, R., Sawai, N., Nakamori, M., Tanaka, Y., Kudo, A., Morikami, S., Iwanaga, N., Akai, G., Shimizu, N., Arakawa, T., Yamada, C., Kitahara, K., Tanaka, K., Ito, Y., Fushinobu, S. and Fujita, K. Identification and characterization of endo-α-, exo-α-, and exo-β-D-arabinofuranosidases degrading lipoarabinomannan and arabinogalactan of mycobacteria. Nat. Commun. 14 (2023) 5803. [PMID: 37726269]