Reaction: Endohydrolysis of α-(1→3)-linkages between 3,6-anhydro-α-L-galactopyranose and β-D-galactopyranose-6-sulfate units in funoran
Glossary: funoran = [→4)-3,6-anhydro-α-L-galactopyranose-(1→3)-β-D-galactopyranose-6-sulfate-(1→]
Other name(s): α-funoranase
Systematic name: funoran endo α-(1,3)-glycanohydrolase
Comments: The enzyme is an endo hydrolase that hydrolyses the α-(1→3) bond in funoran, a polysaccharide produced by red algae of the genus Gloiopeltis. The cleavage point is different from funoran endo-β-hydrolase (EC 3.2.1.222). The enzyme from the marine bacterium Aquimarina sp. AD1 also cleaves the α-(1→3) glycosidic bond between 3,6-anhydro-α-L-galactopyranose and β-D-galactopyranose of agarose (cf. EC 3.2.1.158, α-agarase). The anomeric configuration of the product (configuration retaining or inverting) has not yet been determined.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
References: 1. Zhang, Y., Zheng, L., Liu, G., Shen, J., Chen, G., Mei, X., Chang, Y. and Xue, C. The α-linkage in funoran and agarose could be hydrolyzed by a GH96 family enzyme: Discovery of the α-funoranase. Carbohydr. Polym. 338:122201 (2024). [DOI] [PMID: 38763726]