IUBMB Enzyme Nomenclature


Accepted name: pullulanase

Reaction: Hydrolysis of (1→6)-α-D-glucosidic linkages in pullulan, amylopectin and glycogen, and in the α- and β-limit dextrins of amylopectin and glycogen

Glossary: pullulan = a linear polymer of 1→6-linked maltotriose units.

Other name(s): limit dextrinase (erroneous); amylopectin 6-glucanohydrolase; bacterial debranching enzyme; debranching enzyme; α-dextrin endo-1,6-α-glucosidase; R-enzyme; pullulan α-1,6-glucanohydrolase

Systematic name: pullulan 6-α-glucanohydrolase

Comments: Different from EC (limit dextrinase) in its action on glycogen, and its rate of hydrolysis of limit dextrins. Its action on amylopectin is complete. Maltose is the smallest sugar that it can release from an α-(1→6)-linkage.

Links to other databases: BRENDA, EXPASY, GTD, KEGG, Metacyc, PDB, CAS registry number: 9075-68-7


1. Lee, E.Y.C. and Whelan, W.J. Glycogen and starch debranching enzymes, in Boyer, P.D. (Ed.), The Enzymes, 3rd edn., vol. 5, Academic Press, New York, 1972, pp. 191-234.

2. Bender, H. and Wallenfels, K. Pullulanase (an amylopectin and glycogen debranching enzyme) from Aerobacter aerogenes. Methods Enzymol. 8 (1966) 555-559.

3. Manners, D.J. Observations on the specificity and nomenclature of starch debranching enzymes. J. Appl. Glycosci. 44 (1997) 83-85.

[EC created 1972, modified 1976, modified 2000 (EC created 1972, incorporated 1976)]

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