EC 3.5.1.123

Reaction: N⁵-phenyl-L-glutamine + H₂O = L-glutamate + aniline

Glossary: γ-glutamylanilide = N⁵-phenyl-L-glutamine

Other name(s): atdA2 (gene name)

Systematic name: N⁵-phenyl-L-glutamine amidohydrolase

Comments: The enzyme, characterized from the bacterium Acinetobacter sp. YAA, catalyses the opposite reaction from that catalysed by EC 6.3.1.18, γ-glutamylanilide synthase, which is part of an aniline degradation pathway. Its purpose is likely to maintain a low concentration of N⁵-phenyl-L-glutamine, which is potentially toxic.

Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number:

References:

1. Takeo, M., Ohara, A., Sakae, S., Okamoto, Y., Kitamura, C., Kato, D. and Negoro, S. Function of a glutamine synthetase-like protein in bacterial aniline oxidation via γ-glutamylanilide. J. Bacteriol. 195 (2013) 4406-4414. [PMID: 23893114]