Reaction: a [C-phycoerythrin α-subunit]-Cys82-phycoerythrobilin = apo-[C-phycoerythrin α-subunit] + (3E)-phycoerythrobilin
Other name(s): cpeY (gene name)
Systematic name: [C-phycoerythrin α-subunit]-Cys82-phycoerythrobilin:phycoerythrobilin lyase
Comments: The enzyme, characterized from the cyanobacterium Microchaete diplosiphon, catalyses the attachment of the phycobilin chromophore (3E)-phycoerythrobilin (PEB) to cysteine 82 of the α subunit of the phycobiliprotein C-phycoerythrin. The numbering used here corresponds to the enzyme from Microchaete diplosiphon, and could vary slightly in other organisms. Activity is greatly enhanced in the presence of the chaperone-like protein CpeZ. The reaction could also be catalysed by EC 4.4.1.29, phycobiliprotein cysteine-84 phycobilin lyase, but much less efficiently.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
References:
1. Biswas, A., Boutaghou, M.N., Alvey, R.M., Kronfel, C.M., Cole, R.B., Bryant, D.A. and Schluchter, W.M. Characterization of the activities of the CpeY, CpeZ, and CpeS bilin lyases in phycoerythrin biosynthesis in Fremyella diplosiphon strain UTEX 481. J. Biol. Chem. 286 (2011) 35509-35521. [PMID: 21865169]
2. Kronfel, C.M., Biswas, A., Frick, J.P., Gutu, A., Blensdorf, T., Karty, J.A., Kehoe, D.M. and Schluchter, W.M. The roles of the chaperone-like protein CpeZ and the phycoerythrobilin lyase CpeY in phycoerythrin biogenesis. Biochim Biophys Acta Bioenerg 1860 (2019) S0005-2728(. [PMID: 31173730]