Reaction: a [C-phycoerythrin β-subunit]-Cys48/59-phycoerythrobilin = apo-[C-phycoerythrin α-subunit] + (3E)-phycoerythrobilin
Other name(s): cpeF (gene name)
Systematic name: [C-phycoerythrin β-subunit]-Cys48/59-phycoerythrobilin:phycoerythrobilin lyase
Comments: The enzyme, characterized from the cyanobacterium Microchaete diplosiphon, catalyses the attachment of the phycobilin chromophore (3E)-phycoerythrobilin (PEB) to cysteine 48 and 59 of the β-subunits of the phycobiliprotein C-phycoerythrin. The enzyme first ligates the A ring of PEB to cysteine-48, followed by the attachment of the D ring to cysteine-59. The numbering used here corresponds to the enzyme from Microchaete diplosiphon, and could vary slightly in other organisms. The reaction requires the presence of the chaperone-like protein CpeZ.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
References:
1. Kronfel, C.M., Hernandez, C.V., Frick, J.P., Hernandez, L.S., Gutu, A., Karty, J.A., Boutaghou, M.N., Kehoe, D.M., Cole, R.B. and Schluchter, W.M. CpeF is the bilin lyase that ligates the doubly linked phycoerythrobilin on β-phycoerythrin in the cyanobacterium Fremyella diplosiphon. J. Biol. Chem. 294 (2019) 3987-3999. [PMID: 30670589]