Reaction: ATP + H2O + a kinesin associated with a microtubule at position n = ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)
Other name(s): non-claret disjunctional; ncd (gene name)
Systematic name: kinesin ATP phosphohydrolase (minus-end-directed)
Comments: Kinesins are a family of motor proteins that move unidirectionally along microtubules as they hydrolyse ATP and are involved in organelle movement. This enzyme is similar to EC 5.6.1.3, plus-end-directed kinesin ATPase, but the organization of the different domains differs, resulting in movement in the opposite direction along the microtubules.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
References:
1. McDonald, H.B., Stewart, R.J. and Goldstein, L.S. The kinesin-like ncd protein of Drosophila is a minus end-directed microtubule motor. Cell 63 (1990) 1159-1165. [PMID: 2261638]
2. Chandra, R., Salmon, E.D., Erickson, H.P., Lockhart, A. and Endow, S.A. Structural and functional domains of the Drosophila ncd microtubule motor protein. J. Biol. Chem 268 (1993) 9005-9013. [PMID: 8473343]
3. Lockhart, A. and Cross, R.A. Origins of reversed directionality in the ncd molecular motor. EMBO J. 13 (1994) 751-757. [PMID: 8112290]
4. Henningsen, U. and Schliwa, M. Reversal in the direction of movement of a molecular motor. Nature 389 (1997) 93-96. [PMID: 9288974]
5. Sablin, E.P., CASe, R.B., Dai, S.C., Hart, C.L., Ruby, A., Vale, R.D. and Fletterick, R.J. Direction determination in the minus-end-directed kinesin motor ncd. Nature 395 (1998) 813-816. [PMID: 9796817]