Reaction: a hydroxy-dihydrocinnamate + NAD+ = a hydroxy-trans-cinnamate + NADH + H+
Other name(s): hcrA (gene name); hcrB (gene name); hcrF (gene name); par1 (gene name); crdA (gene name); crdB (gene name)
Systematic name: hydroxycinnamate:NAD+ oxidoreductase (hydroxy-trans-cinnamate-forming)
Comments: Requires FMN. The enzyme, characterized from assorted lactic acid bacteria and the anaerobic bacterium Vibrio ruber, acts on multiple hydroxy-trans-cinnamic acids including 2-coumarate, 3-coumarate, 4-coumarate, ferulate, caffeate and sinapate. It participates in an alternative degradation pathway of hydroxycinnamic acids. The main pathway involves EC 4.1.1.102, phenacrylate decarboxylase, and EC 1.3.1.131, vinylphenol reductase. cf. EC 1.3.1.11, 2-coumarate reductase.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
References:
1. Santamaria, L., Reveron, I., Lopez de Felipe, F., de Las Rivas, B. and Munoz, R. Unravelling the reduction pathway as an alternative metabolic route to hydroxycinnamate decarboxylation in Lactobacillus plantarum. Appl. Environ. Microbiol. 84 (2018) e01123-18. [PMID: 29776925]
2. Gaur, G., Oh, J.H., Filannino, P., Gobbetti, M., van Pijkeren, J.P. and Ganzle, M.G. Genetic determinants of hydroxycinnamic acid metabolism in heterofermentative lactobacilli. Appl. Environ. Microbiol. 86 (2020) e02461-19. [PMID: 31862715]
3. Gaur, G. and Ganzle, M. Biochemical characterization of HcrF from Limosilactobacillus fermentum, a NADH-dependent 2-ene reductase with activity on hydroxycinnamic acids. Lett. Appl. Microbiol. 77 (2024) ovae109. [PMID: 39521943]
4. Bertsova, Y.V., Serebryakova, M.V., Anashkin, V.A., Baykov, A.A. and Bogachev, A.V. A Redox-Regulated, Heterodimeric NADH:cinnamate Reductase in Vibrio ruber. Biochemistry (Mosc.) 89 (2024) 241-256. [PMID: 38622093]