IUBMB Enzyme Nomenclature


Accepted name: 6-phosphofructokinase

Reaction: ATP + β-D-fructofuranose 6-phosphate = ADP + β-D-fructofuranose 1,6-bisphosphate

For diagram of reaction click here

Other name(s): phosphohexokinase; phosphofructokinase I; phosphofructokinase (phosphorylating); 6-phosphofructose 1-kinase; ATP-dependent phosphofructokinase; D-fructose-6-phosphate 1-phosphotransferase; fructose 6-phosphate kinase; fructose 6-phosphokinase; nucleotide triphosphate-dependent phosphofructokinase; phospho-1,6-fructokinase; PFK

Systematic name: ATP:β-D-fructose-6-phosphate 1-phosphotransferase

Comments: The enzyme from rabbit muscle displays absolute stereoselectivity for the β-anomer of D-fructofuranose 6-phosphate [9-11]. D-Tagatose 6-phosphate and sedoheptulose 7-phosphate can act as acceptors. UTP, CTP and ITP can act as donors. Not identical with EC 6-phosphofructo-2-kinase.

Links to other databases: BRENDA, EXPASY, GTD, KEGG, MetaCyc, PDB, CAS registry number: 9001-80-3


1. Racker, E. Spectrophotometric measurement of hexokinase and phosphohexokinase activity. J. Biol. Chem. 167 (1947) 843-854. [PMID: 20287918]

2. Axelrod, B., Saltman, P., Bandurski, R.S. and Baker, R.S. Hexokinase in higher plants. J. Biol. Chem. 197 (1952) 89-96. [PMID: 12981037]

3. Ling, K.H., Pastkau, V., Marcus, F. and Lardy, H.A. Phosphofructokinase. I. Skeletal muscle. Methods Enzymol. 9 (1966) 425-429.

4. Mansour, T.E. Phosphofructokinase. II. Heart muscle. Methods Enzymol. 9 (1966) 430-436.

5. Parmeggiano, A., Luft, J.H., Love, D.S. and Krebs, E.G. Crystallization and properties of rabbit skeletal muscle phosphofructokinase. J. Biol. Chem. 241 (1966) 4625-4637. [PMID: 4224472]

6. Sols, A. and Salas, M.L. Phosphofructokinase. III. Yeast. Methods Enzymol. 9 (1966) 436-442.

7. Odeide, R., Guilloton, M., Dupuis, B., Ravon, D. and Rosenberg, A.J. Study of an enzyme allosteric to 2 substrates: phosphofructokinase of rat muscle. I. Preparation and crystallization of the enzyme. Bull. Soc. Chim. Biol. 50 (1968) 2023-2033. [PMID: 4237772]

8. Uyeda, K. and Kurooka, S. Crystallization and properties of phosphofructokinase from Clostridium pasteurianum. J. Biol. Chem. 245 (1970) 3315-3324. [PMID: 4248230]

9. Fishbein, R., Benkovic, P.A., Schray, K.J., Siewers, I.J., Steffens, J.J. and Benkovic, S.J. Anomeric specificity of phosphofructokinase from rabbit muscle. J. Biol. Chem. 249 (1974) 6047-6051. [PMID: 4278654]

10. Wurster, B. and Hess, B. Anomeric specificity of fructose-6-phosphate kinase (EC from rabbit muscle. FEBS Lett. 38 (1974) 257-260. [PMID: 4277364]

11. Koerner, T.A., Jr., Younathan, E.S., Ashour, A.L. and Voll, R.J. The fructose 6-phosphate site of phosphofructokinase. I. Tautomeric and anomeric specificity. J. Biol. Chem. 249 (1974) 5749-5754. [PMID: 4278316]

[EC created 1961, modified 2021]

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