Reaction: ATP + N-acetyl-D-hexosamine = ADP + N-acetyl-α-D-hexosamine 1-phosphate
Other name(s): NahK; LnpB; N-acetylgalactosamine/N-acetylglucosamine 1-kinase
Systematic name: ATP:N-acetyl-D-hexosamine 1-phosphotransferase
Comments: This enzyme is involved in the lacto-N-biose I/galacto-N-biose degradation pathway in the probiotic bacterium Bifidobacterium longum. Differs from EC 18.104.22.168, N-acetylgalactosamine kinase, as it can phosphorylate both N-acetylgalactosamine and N-acetylglucosamine at similar rates. Also has some activity with N-acetyl-D-mannosamine, D-talose and D-mannose as substrate. ATP can be replaced by GTP or ITP but with decreased enzyme activity. Requires a divalent cation, with Mg2+ resulting in by far the greatest stimulation of enzyme activity.
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number:
1. Nishimoto, M. and Kitaoka, M. Identification of N-acetylhexosamine 1-kinase in the complete lacto-N-biose I/galacto-N-biose metabolic pathway in Bifidobacterium longum. Appl. Environ. Microbiol. 73 (2007) 6444-6449. [PMID: 17720833]