Reaction: tRNAn+1 + phosphate = tRNAn + a nucleoside diphosphate
Other name(s): phosphate-dependent exonuclease; RNase PH; ribonuclease PH
Systematic name: tRNA:phosphate nucleotidyltransferase
Comments: Brings about the final exonucleolytic trimming of the 3'-terminus of tRNA precursors in Escherichia coli by a phosphorolysis, producing a mature 3'-terminus on tRNA and nucleoside diphosphate. Not identical with EC 188.8.131.52 polyribonucleotide nucleotidyltransferase.
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number: 116412-36-3
1. Cudny, H. and Deutscher, M.P. 3' processing of tRNA precursors in ribonuclease-deficient Escherichia coli. Development and characterization of an in vitro processing system and evidence for a phosphate requirement. J. Biol. Chem. 263 (1988) 1518-1523. [PMID: 3275667]
2. Deutscher, M.P., Marshall, G.T. and Cudny, H. RNase PH: an Escherichia coli phosphate-dependent nuclease distinct from polynucleotide phosphorylase. Proc. Natl. Acad. Sci. USA 85 (1988) 4710-4714. [PMID: 2455297]