IUBMB Enzyme Nomenclature


Accepted name: tRNA pseudouridine32 synthase

Reaction: tRNA uridine32 = tRNA pseudouridine32

For diagram of mechanism click here.

Other name(s): RluA (ambiguous); pseudouridine synthase RluA (ambiguous)

Systematic name: tRNA-uridine32 uracil mutase

Comments: The dual-specificity enzyme also catalyses the formation of pseudouridine746 in 23S rRNA [5]. cf. EC (23S rRNA pseudouridine746 synthase).

Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number:


1. Hoang, C., Chen, J., Vizthum, C.A., Kandel, J.M., Hamilton, C.S., Mueller, E.G. and Ferre-D'Amare, A.R. Crystal structure of pseudouridine synthase RluA: indirect sequence readout through protein-induced RNA structure. Mol. Cell 24 (2006) 535-545. [PMID: 17188032]

2. Spedaliere, C.J., Hamilton, C.S. and Mueller, E.G. Functional importance of motif I of pseudouridine synthases: mutagenesis of aligned lysine and proline residues. Biochemistry 39 (2000) 9459-9465. [PMID: 10924141]

3. Raychaudhuri, S., Niu, L., Conrad, J., Lane, B.G. and Ofengand, J. Functional effect of deletion and mutation of the Escherichia coli ribosomal RNA and tRNA pseudouridine synthase RluA. J. Biol. Chem. 274 (1999) 18880-18886. [PMID: 10383384]

4. Ramamurthy, V., Swann, S.L., Spedaliere, C.J. and Mueller, E.G. Role of cysteine residues in pseudouridine synthases of different families. Biochemistry 38 (1999) 13106-13111. [PMID: 10529181]

5. Wrzesinski, J., Nurse, K., Bakin, A., Lane, B.G. and Ofengand, J. A dual-specificity pseudouridine synthase: an Escherichia coli synthase purified and cloned on the basis of its specificity for psi746 in 23S RNA is also specific for Ψ32 in tRNA(phe). RNA 1 (1995) 437-448. [PMID: 7493321]

[EC created 2011]

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